Example 04: Application: Qiime Ecosystem

Goal: Demonstrate importing the large qiime application ecosystem.


In the following examples, we assume you already have a miniconda3 module already loaded.


Can I use a Channel

Not from the channels I have configured.

[]$ conda search qiime Loading channels: done No match found for: qiime. Search: *qiime* # Name Version Build Channel r-qiimer 0.9.4 r3.3.2_0 conda-forge ... r-qiimer 0.9.4 r43hc72bb7e_1005 conda-forge

Only older versions in bioconda channel.

[]$ conda search bioconda::qiime Loading channels: done # Name Version Build Channel qiime 1.8.0 py27_0 bioconda ... qiime 1.9.1 py_3 bioconda

Using a Definition File

From Install QIIME2 within a conda environment:

[]$ pwd /project/<project-name>/<username>/software []$ mkdir qiime []$ cd qiime/ []$ wget https://data.qiime2.org/distro/amplicon/qiime2-amplicon-2024.5-py38-linux-conda.yml []$ conda env create -p amplicon-2024.5 --file qiime2-amplicon-2024.5-py38-linux-conda.yml []$ conda activate /cluster/medbow/project/<project-name>/<username>/software/qiime/amplicon-2024.5 (/cluster/medbow/project/<project-name>/<username>/software/qiime/amplicon-2024.5) []$ qiime info System versions Python version: 3.9.19 QIIME 2 release: 2024.5 QIIME 2 version: 2024.5.0 q2cli version: 2024.5.0 ...

What’s in this Qiime Environment?

This environment contains qiime commands, R and libraries, Python and packages…


How Large is this Environment?

This is just the qiime environment. It does not include all the packages that were downloaded.

What about all the other environments we have created?


Cleaning Up